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imagequant tl 1d gel analysis program  (Cytiva Europe)


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    Structured Review

    Cytiva Europe imagequant tl 1d gel analysis program
    Imagequant Tl 1d Gel Analysis Program, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/imagequant+analysis+program/pm30843284-77-6-12
    Average 86 stars, based on 1 article reviews
    imagequant tl 1d gel analysis program - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Western Blot:

    Article Title: Maternal high fat–high energy diet alters metabolic factors in the non‐human primate fetal heart
    Article Snippet: Enhanced chemiluminescence using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific) was used to detect the bands on the blots. .. The western blot was imaged using ImageQuant LAS 4000 (GE Healthcare Life sciences) and the protein abundance was quantified by densitometry using ImageQuant analysis software (GE Healthcare Life Sciences) and normalised to either β-actin or Ponceau S (total protein stain) as previously described (Darby et al., 2018; Lie et al., 2014; Soo et al., 2012). .. Due to the amount of frozen tissue available, tissue hormone (cortisol, cortisone, 11-deoxycortisone, triiodothyronine (T3) and thyroxine (T4)) concentrations in a subset of animals (control diet, n= 2 F, 5M;HF-HED, n = 5 F, 6 M) were determined by liquid chromatography (LC; Shimadzu Nexera XR, Shimadzu, Japan) coupled to a SCIEX 6500 Triple-Quad system (MS/MS; SCIEX, Framingham, MA, USA) using an adapted protocol (Dimasi et al., 2023b; Lock et al., 2023; McBride et al., 2020).

    Article Title: Citrus sunki Peel Extract, Containing Nobiletin and Tangeretin, Enhances Proliferation and Differentiation in 3T3-L1 Adipocytes
    Article Snippet: After washing four times with TBST again (10min each wash), Clarity western ECL substrate (Bio-Rad, USA) was used to detect proteins. .. Band images were taken using a Western blot imager (AmershamTM ImageQuantTM 800, Cytiva, Amersham, England), and densitometry was quantifed using the ImageQuant TL analysis software (Cytiva). ..

    Quantitative Proteomics:

    Article Title: Maternal high fat–high energy diet alters metabolic factors in the non‐human primate fetal heart
    Article Snippet: Enhanced chemiluminescence using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific) was used to detect the bands on the blots. .. The western blot was imaged using ImageQuant LAS 4000 (GE Healthcare Life sciences) and the protein abundance was quantified by densitometry using ImageQuant analysis software (GE Healthcare Life Sciences) and normalised to either β-actin or Ponceau S (total protein stain) as previously described (Darby et al., 2018; Lie et al., 2014; Soo et al., 2012). .. Due to the amount of frozen tissue available, tissue hormone (cortisol, cortisone, 11-deoxycortisone, triiodothyronine (T3) and thyroxine (T4)) concentrations in a subset of animals (control diet, n= 2 F, 5M;HF-HED, n = 5 F, 6 M) were determined by liquid chromatography (LC; Shimadzu Nexera XR, Shimadzu, Japan) coupled to a SCIEX 6500 Triple-Quad system (MS/MS; SCIEX, Framingham, MA, USA) using an adapted protocol (Dimasi et al., 2023b; Lock et al., 2023; McBride et al., 2020).

    Software:

    Article Title: Maternal high fat–high energy diet alters metabolic factors in the non‐human primate fetal heart
    Article Snippet: Enhanced chemiluminescence using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific) was used to detect the bands on the blots. .. The western blot was imaged using ImageQuant LAS 4000 (GE Healthcare Life sciences) and the protein abundance was quantified by densitometry using ImageQuant analysis software (GE Healthcare Life Sciences) and normalised to either β-actin or Ponceau S (total protein stain) as previously described (Darby et al., 2018; Lie et al., 2014; Soo et al., 2012). .. Due to the amount of frozen tissue available, tissue hormone (cortisol, cortisone, 11-deoxycortisone, triiodothyronine (T3) and thyroxine (T4)) concentrations in a subset of animals (control diet, n= 2 F, 5M;HF-HED, n = 5 F, 6 M) were determined by liquid chromatography (LC; Shimadzu Nexera XR, Shimadzu, Japan) coupled to a SCIEX 6500 Triple-Quad system (MS/MS; SCIEX, Framingham, MA, USA) using an adapted protocol (Dimasi et al., 2023b; Lock et al., 2023; McBride et al., 2020).

    Article Title: Citrus sunki Peel Extract, Containing Nobiletin and Tangeretin, Enhances Proliferation and Differentiation in 3T3-L1 Adipocytes
    Article Snippet: After washing four times with TBST again (10min each wash), Clarity western ECL substrate (Bio-Rad, USA) was used to detect proteins. .. Band images were taken using a Western blot imager (AmershamTM ImageQuantTM 800, Cytiva, Amersham, England), and densitometry was quantifed using the ImageQuant TL analysis software (Cytiva). ..

    Article Title: T-cell acute lymphoblastic leukemia progression is supported by inflammatory molecules including Hepatocyte Growth factor
    Article Snippet: Chemiluminescence signal was quantified using Gbox (Syngene) device. .. The analysis of the spots intensities and background noise removal was performed using ImageQuant TL software (Version 8.1, GE Healthcare). ..

    Article Title: Loss of the lysosomal lipid flippase ATP10B leads to progressive dopaminergic neurodegeneration and Parkinsonian motor deficits
    Article Snippet: Bands were visualised using Clarity Western ECL (Bio-Rad) and developed with a GE ImageQuant 800 (GE Healthcare). .. Densitometric analysis was performed using ImageQuant software (GE Healthcare). .. The human induced pluripotent stem cells (iPSCs) BJ-SiPS line was cultured using StemflexTM medium (ThermoFisher).

    Article Title: Involvement of mitogen- and stress-activated protein kinase 1 in BMP-6–induced chondrocyte differentiation
    Article Snippet: The membranes were then probed with the indicated primary antibodies, which were detected using horseradish peroxidase–conjugated secondary antibodies and a chemiluminescent substrate (cat. no. T7101A, Western BLoT Quant HRP Substrate, TAKARA). .. The intensity of each band was measured using ImageQuant TL analysis software (Cytiva) after each gel image was captured with the ImageQuant LAS 4000 mini (Amersham). ..

    Article Title: Structural and biochemical characterization of the C‐terminal region of the human RTEL 1 helicase
    Article Snippet: .. Fluorescent labeled substrates were detected by fluorescent scanner (ImageQuant, GE Healthcare) and quantification of protein bound nucleic acid was performed with ImageQuant image analysis software (GE Healthcare). .. Each experiment has been repeated three times and plotted using Prism 8 (Graphpad).

    Article Title: Organ transformation by environmental disruption of protein integrity and epigenetic memory in Drosophila
    Article Snippet: .. Membranes were imaged using the IQ800 imaging system (Cytiva) and band densities were quantified using ImageQuant TL analysis software. .. The Hsp83 protein was detected using rabbit anti-Hsp83 primary antibody (1:480 dilution, 4°C, O/N, DSHB, obtained from Paro lab (Tariq and colleagues) and HRP-conjugated goat anti-rabbit IgG secondary antibody (1:20,000 dilution, RT, 1 h, Jackson ImmunoResearch, catalog ##115-035-062).

    Article Title: Prenatal cigarette smoke exposure sensitizes acetaminophen-induced liver injury by modulating miR-34a-5p in male offspring mice
    Article Snippet: Following four washes with TBS-T, these membranes were visualized using a Western ECL Kit (LPS solution, Daejeon, Korea) and captured with ImageQuant LAS 500 (GE Healthcare Life Science, PA, United States). .. Image analysis to determine relative intensity was conducted using ImageQuant TL software version 8.1 (GE Healthcare Life Science). ..

    Staining:

    Article Title: Maternal high fat–high energy diet alters metabolic factors in the non‐human primate fetal heart
    Article Snippet: Enhanced chemiluminescence using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific) was used to detect the bands on the blots. .. The western blot was imaged using ImageQuant LAS 4000 (GE Healthcare Life sciences) and the protein abundance was quantified by densitometry using ImageQuant analysis software (GE Healthcare Life Sciences) and normalised to either β-actin or Ponceau S (total protein stain) as previously described (Darby et al., 2018; Lie et al., 2014; Soo et al., 2012). .. Due to the amount of frozen tissue available, tissue hormone (cortisol, cortisone, 11-deoxycortisone, triiodothyronine (T3) and thyroxine (T4)) concentrations in a subset of animals (control diet, n= 2 F, 5M;HF-HED, n = 5 F, 6 M) were determined by liquid chromatography (LC; Shimadzu Nexera XR, Shimadzu, Japan) coupled to a SCIEX 6500 Triple-Quad system (MS/MS; SCIEX, Framingham, MA, USA) using an adapted protocol (Dimasi et al., 2023b; Lock et al., 2023; McBride et al., 2020).

    Labeling:

    Article Title: Structural and biochemical characterization of the C‐terminal region of the human RTEL 1 helicase
    Article Snippet: .. Fluorescent labeled substrates were detected by fluorescent scanner (ImageQuant, GE Healthcare) and quantification of protein bound nucleic acid was performed with ImageQuant image analysis software (GE Healthcare). .. Each experiment has been repeated three times and plotted using Prism 8 (Graphpad).

    Imaging:

    Article Title: Organ transformation by environmental disruption of protein integrity and epigenetic memory in Drosophila
    Article Snippet: .. Membranes were imaged using the IQ800 imaging system (Cytiva) and band densities were quantified using ImageQuant TL analysis software. .. The Hsp83 protein was detected using rabbit anti-Hsp83 primary antibody (1:480 dilution, 4°C, O/N, DSHB, obtained from Paro lab (Tariq and colleagues) and HRP-conjugated goat anti-rabbit IgG secondary antibody (1:20,000 dilution, RT, 1 h, Jackson ImmunoResearch, catalog ##115-035-062).



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